4Misc_Start2#4Platform@Q9VersionCheck xHH@Rg(HHdh xHH@Rg(HHdh x HH@Rg(HHdh ^Graph*2WDashSettings#  !4 4 4 4 4 4 vhomeBBdG:cikkek:cikk gödöllő:IgorFiles:RecentWindowsbaseEM-UFHA-ALGraph0:ZP vs cUFHA_ZP;base vs cUFHA_ZP;...Graph1:EM_UFHA vs cUFHA_EM;W vs cUFHA_W;...WW-KClWaveSelectorWidget 4Misc_End2#4XOPState_Start2#4XOPState_End2#|X_Az~T$@V_FlagV_chisq1\v?V_numNaNsV_numINFsV_npnts@V_nterms@V_nheldV_startRowV_endRow@V_startColV_endColV_startLayerV_endLayerV_startChunkV_endChunkS_waveNames wave0;wave1;S_pathamesS_fileName ClipboardK •Edit as "W-KCl" •Rename wave0,'c(KCl)' •Rename wave1,'W(KCl)' •Display 'W(KCl)' vs 'c(KCl)' •ModifyGraph margin(left)=70,margin(bottom)=70,margin(right)=30,margin(top)=30,width=300,height=300 •ModifyGraph log(bottom)=1,fStyle(bottom)=1,fSize(bottom)=15,axThick(bottom)=1.2 •DeletePoints 0,1, 'c(KCl)','W(KCl)' •DeletePoints 7,1, 'c(KCl)','W(KCl)' •Label left "\\Z24Stability ratio ";DelayUpdate •ModifyGraph log=1,minor(left)=1,fStyle=1,fSize=15,axThick=1.2,btLen(left)=3,stLen(left)=2 •Label bottom "\\Z24KCl concentration (mM)" •ModifyGraph marker=16,msize=5,rgb=(32768,32770,65535),useMrkStrokeRGB=1 •SetAxis bottom *,1000 •ModifyGraph rgb=(1,39321,39321) •Edit as "plus points" •AppendToGraph wave1 vs wave0 •Rename wave0,wplus •Rename wplus,'c plus' •Rename wave1,'w plus' •DeletePoints 0,2, 'c plus','w plus' •RemoveFromGraph 'w plus' •TextBox/C/N=text0/F=0/A=MC "\\f01\\Z16[AL\\B110\\M\\Z16] = 10 ppm\r" •ModifyGraph rgb=(49151,53155,65535) •SavePICT/E=-6/B=72 as "W-KCL-AL.jpg" •DeletePoints 8,2, 'W(KCl)' •DeletePoints 8,8, 'W(KCl)' •DeletePoints 8,4, 'W(KCl)' •ModifyGraph rgb=(49151,49152,65535) •SetAxis left *,2000 •SavePICT/E=-6/B=72 •TextBox/C/N=text0 "\\f01\\Z14[AL\\B110\\M\\Z16] = 10 ppm\r" •SavePICT/E=-6/B=72 •Rename 'W(KCl)',ZP_NaCl •Rename 'c(KCl)',cNaCl •SetAxis left -30,30;DelayUpdate •ModifyGraph log(left)=0,minor=0,fStyle=0,fSize=10,axThick=1,btLen=3,stLen=1;DelayUpdate •Label left "Zeta Potential (mV)";DelayUpdate •Label bottom "NaCl concentration (mM)" •Rename wave0,e_NaCl •ModifyGraph margin(left)=44,margin(bottom)=44,margin(right)=5,margin(top)=5,width=185,height=185 •TextBox/C/N=text0 "NaCl\r\r" •SetAxis left -10,30 •ModifyGraph marker=19,msize=3,rgb=(44253,29492,58982);DelayUpdate •ErrorBars/RGB=(0,0,0) ZP_NaCl Y,wave=(e_NaCl,e_NaCl) •SetAxis left -10,50 •InsertPoints 5,1, cNaCl,ZP_NaCl,e_NaCl •InsertPoints 3,1, cNaCl,ZP_NaCl,e_NaCl •SetAxis bottom 7,1000 •Rename cNaCl,cufha •Rename ZP_NaCl,ZP •Rename e_NaCl,err •SetAxis bottom 1,1000 •DeletePoints 2,4, cufha,ZP,err •DeletePoints 2,1, cufha,ZP,err •DeletePoints 6,1, cufha,ZP,err •SetAxis bottom 1,10000 •SetAxis left -10,30 •Rename wave0,abse •Rename abse,base •AppendToGraph base vs cufha •ModifyGraph mode(base)=0,lstyle(base)=3,rgb(base)=(30583,30583,30583) •SetAxis left -20,30 •DeletePoints 7,1, cufha,ZP,err,base •SetAxis left -30,30 •InsertPoints 6,1, cufha,ZP,err,base •InsertPoints 8,1, cufha,ZP,err,base •DeletePoints 8,1, cufha,ZP,err,base •SavePICT/E=-6/B=72 as "ZP-UFHA-AL(25)-NaCl.jpg" •DeletePoints 0,2, cufha,ZP,err,base •SetAxis bottom 30,10000 •CurveFit/TBOX=768 HillEquation ZP /X=cufha /D Fit converged properly fit_ZP= W_coef[0]+(W_coef[1]-W_coef[0])*(fitX_ZP[p]^W_coef[2]/(fitX_ZP[p]^W_coef[2]+W_coef[3]^W_coef[2])) W_coef={19.01,-38.1,1.0431,3182.9} V_chisq= 6.90456;V_npnts= 7;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 6; W_sigma={1.55,9.51,0.25,1.12e+03} Coefficient values ± one standard deviation base = 19.01 ± 1.55 max = -38.1 ± 9.51 rate = 1.0431 ± 0.25 xhalf = 3182.9 ± 1.12e+03 •TextBox/K/N=CF_ZP •Label bottom "UFHA concentration (mM)" •InsertPoints 0,1, cufha,ZP,err,base •SetAxis bottom 10,10000 •SetAxis left -30,40 •CurveFit/TBOX=768 HillEquation ZP /X=cufha /D 40 iterations with no convergence fit_ZP= W_coef[0]+(W_coef[1]-W_coef[0])*(fitX_ZP[p]^W_coef[2]/(fitX_ZP[p]^W_coef[2]+W_coef[3]^W_coef[2])) W_coef={57.016,-61.03,0.29052,1332} V_chisq= 203.256;V_npnts= 8;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 7; W_sigma={112,234,0.879,1.1e+04} Coefficient values ± one standard deviation base = 57.016 ± 112 max = -61.03 ± 234 rate = 0.29052 ± 0.879 xhalf = 1332 ± 1.1e+04 •TextBox/K/N=CF_ZP •DeletePoints 0,1, cufha,ZP,err,base •CurveFit/TBOX=768 HillEquation ZP /X=cufha /D Fit converged properly fit_ZP= W_coef[0]+(W_coef[1]-W_coef[0])*(fitX_ZP[p]^W_coef[2]/(fitX_ZP[p]^W_coef[2]+W_coef[3]^W_coef[2])) W_coef={19.01,-38.1,1.0431,3182.9} V_chisq= 6.90456;V_npnts= 7;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 6; W_sigma={1.55,9.51,0.25,1.12e+03} Coefficient values ± one standard deviation base = 19.01 ± 1.55 max = -38.1 ± 9.51 rate = 1.0431 ± 0.25 xhalf = 3182.9 ± 1.12e+03 •TextBox/K/N=CF_ZP •SetAxis bottom 30,10000 •SetAxis bottom 0.03,1 •SetAxis bottom 0.03,10 •CurveFit/TBOX=768 HillEquation ZP /X=cufha /D Fit converged properly fit_ZP= W_coef[0]+(W_coef[1]-W_coef[0])*(fitX_ZP[p]^W_coef[2]/(fitX_ZP[p]^W_coef[2]+W_coef[3]^W_coef[2])) W_coef={19.01,-38.1,1.0431,3.1829} V_chisq= 6.90456;V_npnts= 7;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 6; W_sigma={1.55,9.51,0.25,1.12} Coefficient values ± one standard deviation base = 19.01 ± 1.55 max = -38.1 ± 9.51 rate = 1.0431 ± 0.25 xhalf = 3.1829 ± 1.12 •TextBox/K/N=CF_ZP •ModifyGraph rgb(fit_ZP)=(44253,29492,58982) •TextBox/C/N=text1/F=0/B=1 "[AL] = 25 ppm" •SavePICT/E=-6/B=72 as "ZP-AL25-UFHA-NaCl1.jpg" •SetAxis bottom 0.01,10 •Edit as "base" •DeletePoints 0,7, base •Rename wave0,basex •Rename wave1,basey •AppendToGraph basey vs basex •ModifyGraph mode(basey)=0,lstyle(basey)=3,rgb(basey)=(34952,34952,34952) •ModifyGraph rgb(ZP)=(1,39321,39321),rgb(fit_ZP)=(1,39321,39321) •SavePICT/E=-2 •SavePICT/E=-2 •ModifyGraph mode(fit_ZP)=3,marker(fit_ZP)=16,msize(fit_ZP)=3,rgb(fit_ZP)=(1,34817,52428) •ModifyGraph mode(fit_ZP)=0 •ModifyGraph marker(ZP)=16,rgb(ZP)=(1,34817,52428) •Label bottom "UFHA Concentration (mM)" •TextBox/K/N=text1 •TextBox/K/N=text0 •ModifyGraph mode(fit_ZP)=3,rgb(fit_ZP)=(52428,17472,1) •ModifyGraph mode(fit_ZP)=0 •ModifyGraph rgb(ZP)=(52428,17472,1) •ModifyGraph marker(ZP)=19,rgb(ZP)=(65535,1799,24158) •ModifyGraph rgb(fit_ZP)=(65535,1799,24158) •SavePICT/E=-2 •ModifyGraph rgb(ZP)=(65535,3855,13107),rgb(fit_ZP)=(65535,3855,13107) •Edit as "W" •Rename wave0,cUFHAW •Rename cUFHAW,cUFHA_W •Rename wave1,W •Rename cufha,cUFHA_ZP •AppendToGraph/R/T W vs cUFHA_W •ModifyGraph margin(right)=44 •SetAxis right 1,50;DelayUpdate •ModifyGraph log(top)=1,btLen=3,stLen=1;DelayUpdate •Label right "Stability Ratio" •ModifyGraph mode(W)=3,marker(W)=16,useMrkStrokeRGB(W)=1 •ModifyGraph rgb(W)=(0,0,65535) •ModifyGraph rgb(W)=(1,26221,39321) •SavePICT/E=-2 •SetAxis right 1,1000;DelayUpdate •ModifyGraph log(right)=1 •SavePICT/E=-2 •Rename err,err_ZP •Edit as "EM-UFHA-AL" •Display EM_UFHA vs cUFHA_EM •Label left "Electrophoretic Mobility\r(10\\S-8\\Mm\\S2\\MV\\S-1\\Ms\\S-1\\M)";DelayUpdate •ModifyGraph mirror(left)=2,btLen(left)=3,stLen(left)=1 •SetAxis left -2,2 •ModifyGraph log(bottom)=1,mirror=2,btLen=3,stLen=1;DelayUpdate •Label bottom "UFHA Concentration (mM)" •ModifyGraph margin(left)=44,margin(bottom)=44,margin(right)=44,margin(top)=5,width=185,height=185 •AppendToGraph/R/T W vs cUFHA_W •ModifyGraph log(right)=1,log(top)=1,btLen(right)=3,stLen(right)=1;DelayUpdate •Label right "Stability Ratio";DelayUpdate •SetAxis right 1,100 •ModifyGraph mode(EM_UFHA)=3,marker(EM_UFHA)=19,msize(EM_UFHA)=3,useMrkStrokeRGB(EM_UFHA)=1;DelayUpdate •ErrorBars/RGB=(0,0,0) EM_UFHA Y,wave=(err_EM,err_EM) •ModifyGraph rgb(EM_UFHA)=(65535,3855,13107) •ModifyGraph mode=3,msize=3,useMrkStrokeRGB=1,marker(W)=16,rgb(W)=(1,26221,39321) •SetAxis right 1,100 •AppendToGraph basey vs basex •ModifyGraph lstyle(basey)=3,rgb(basey)=(34952,34952,34952) •SetAxis left -30,30 •SetAxis left -30,20 •ShowInfo •CurveFit Sigmoid EM_UFHA /X=cUFHA_EM /D 40 iterations with no convergence fit_EM_UFHA= W_coef[0] + W_coef[1]/(1+exp(-(fitX_EM_UFHA[p]-W_coef[2])/W_coef[3])) W_coef={13.959,-15.929,-3.327,2.564} V_chisq= 0.0616185;V_npnts= 7;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 6; W_sigma={59.7,59.8,14.5,1.89} Coefficient values ± one standard deviation base = 13.959 ± 59.7 max = -15.929 ± 59.8 xhalf = -3.327 ± 14.5 rate = 2.564 ± 1.89 •ModifyGraph rgb(fit_EM_UFHA)=(65535,3855,13107) •SavePICT/E=-2 •SavePICT/E=-2 •SavePICT/E=-2 !]xXWŮ,cUFHA_ZP????Q??333333??@@$@xWǮYf'ZP????.j2@g>0@mk/@YX@yĎH\9`eW汷c plus????.@N@b@@`xW汷w plus????@??Q@{xW9&Lerr_ZP????ϐnT?S8?2,#?qgs?l*e?{T}P??چ@ZWK&Lbase????7``WW_coef????Fu+@2/Ed. Cz@I ɀ\Wf'f'fit_ZPŦ????Q?/uZ22@p9G2@þJ2@2@uX2@{4z2@+x~|2@ tcx2@z()t2@f'o2@E2Pk2@Bدf2@a2@ت]2@Қ\W2@JhR2@`_M2@p|G2@';%B2@zVG<2@ I<62@%v02@?͘)2@st"2@Z]02@.2@ 2d 2@ǁ2@'1@}b1@T1@$׆1@eq'1@cBq1@2%1@11@|x߸1@K1@8bڪ1@­U1@qDpA1@"1@ӑx1@l1@Rtʼn`1@ES1@Zѩ&G1@y 91@l",1@Y7P1@31@DoPԸ1@ i0@T0@'Lu0@' 0@410@„ 0@/RQ0@pIy0@z!f0@@,noR0@0>0@A:a)0@`0@F/@4/@Sp/@JUڥp/@5#?/@+tp /@6.@kK諤.@f)n.@o26.@%"-@G-@"u9-@2R]lG-@6Y-@qb,@½gق,@RbP>,@mr+@\b+@l}9d+@D+@*@N .z*@ )*@" "B)@ڭc_)@c,k')@^(@/q(@lOb(@J'@g0O'@ &M}&@!e+&@M"&@SO%@[{2?%@q$@"C[$@b#@nm#@.I"@u"@-(c9!@@ t!@B]tO @Beg @(4@ph8@| @Ha@x9@n2 @\'@ t@3{b@A@@$@f4L@P!`9@x5 @0\ @*QK @X@ @t@Xz(%V)ft2*.+ +V+v24,# a-m.}QB/2Zb 0ؤӊt0 n0ȀD1it:1#pqM2;Ru2/G2Y<3^3l3_4ld^4 '5Őg7rx5y+57-606!|o6d477WG7"/08?1 8]q"8fit_ZP= W_coef[0]+(W_coef[1]-W_coef[0])*(fitX_ZP[p]^W_coef[2]/(fitX_ZP[p]^W_coef[2]+W_coef[3]^W_coef[2])) W_coef={19.01,-38.1,1.0431,3.1829} V_chisq= 6.90456;V_npnts= 7;V_numNaNs= 0;V_numINFs= 0; V_startRow= 0;V_endRow= 6; W_sigma={1.55,9.51,0.25,1.12} Coefficient values ± one standard deviation base = 19.01 ± 1.55 max = -38.1 ± 9.51 rate = 1.0431 ± 0.25 xhalf = 3.1829 ± 1.12 O]Wf'f'fitX_ZP????Q??F?G H? . 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H3?﫛?af*?N?Hjx? k?^?@{yQ?x C?݉~p5?X[&?_?}H?&\?L?- ?@6v?؁??8{8? &*Z{?eg?3רR?Pg=? O|(???/?]?7$?ഀP?o}?wb?,z_F?`ŝM*?@I*J ?h?Xnx?ȷMW?=?pոl?--J?bˇ&?Xߴ?%d?T)!?ErDo?ZX?ӟ&?a/?ȱ1?s yU?DMn?A!?ׄI?k O? ?9O$k?o [+?OL\?Xӳ?4O"?i?"oH?Zl.?_bp ?\#+?=?I?!o/l ?$':?U5ԕ ?Ɋ?hrF?|2?|~?!?m]R?v?'kX(?@J'6?H~қ?}jB^?=2%?%?L`?5?9ñĂ? j#j?8U.,?/D5Ҧ?%?? ,?#>\Ͷ?.Z D??חkFm?Sq-?eϪ?5K?.?ٿ. ?^QJV?U?K?}?'?q_Y?^7^)?ޕd??Vz3?hL?ZM''K?iH?cE?m?qO?1tY.?Ii?I?&Ce? ᮡt0?FUo#?\v?Z ?c(=>P?pH?K`>?ZE?o믿b?9qDV?yL_?;s6?0O?l?? ky?I[j?_?6>g?\D?Uh@Dr?9"?5ʎ?LPx#?|ڷλ?\?Y?C@ǥy@WL+@-w)@OX @{Uh`4b @q;F* @lF @)+` @q{¦ @ԣCJ@ o@ .@@i5Gw@*<7'@ gM@*g/@Jƹ@HG@gQ@,@Mp@8 @#Q@q6lO@![/&@Q ͦ@L('Z@CW@@VI@BM^@sJ-@Fb@@@׼S@LM @` @JKH!@Pk+!@:OR"@T͢"@~rl#@$@* // Platform=WindowsNT, IGORVersion=9.010, architecture=Intel, systemTextEncoding="Windows-1250", historyTextEncoding="UTF-8", procwinTextEncoding="UTF-8", recreationTextEncoding="UTF-8", build=39200 #pragma TextEncoding = "UTF-8" Silent 101 // use | as bitwise or -- not comment. DefaultFont "Arial" MoveWindow/P 5.25,44.75,705,500 Table2() Table3() Table1() Table0() MoveWindow/C 7.5,582.5,697.5,720.5 Graph0() Graph1() KillStrings/Z root:gWMSetNextTextFilesTextEncoding Window Graph1() : Graph PauseUpdate; Silent 1 // building window... Display /W=(870,290,1142.25,523.25) EM_UFHA vs cUFHA_EM AppendToGraph/R/T W vs cUFHA_W AppendToGraph basey vs basex AppendToGraph fit_EM_UFHA vs fitX_EM_UFHA ModifyGraph margin(left)=44,margin(bottom)=44,margin(top)=5,margin(right)=44,width=185 ModifyGraph height=185 ModifyGraph mode(EM_UFHA)=3,mode(W)=3 ModifyGraph marker(EM_UFHA)=19,marker(W)=16 ModifyGraph lStyle(basey)=3 ModifyGraph rgb(EM_UFHA)=(65535,3855,13107),rgb(W)=(1,26221,39321),rgb(basey)=(34952,34952,34952) ModifyGraph rgb(fit_EM_UFHA)=(65535,3855,13107) ModifyGraph msize(EM_UFHA)=3,msize(W)=3 ModifyGraph useMrkStrokeRGB(EM_UFHA)=1,useMrkStrokeRGB(W)=1 ModifyGraph log(bottom)=1,log(right)=1,log(top)=1 ModifyGraph btLen(left)=3,btLen(bottom)=3,btLen(right)=3 ModifyGraph stLen(left)=1,stLen(bottom)=1,stLen(right)=1 Label left "Electrophoretic Mobility\r(10\\S-8\\Mm\\S2\\MV\\S-1\\Ms\\S-1\\M)" Label bottom "UFHA Concentration (mM)" Label right "Stability Ratio" SetAxis left -2,2 SetAxis right 1,100 ErrorBars/RGB=(0,0,0) EM_UFHA Y,wave=(err_EM,err_EM) Cursor/P A EM_UFHA 0;Cursor/P B EM_UFHA 6 ShowInfo EndMacro Window Graph0() : Graph PauseUpdate; Silent 1 // building window... Display /W=(120,308.75,392.25,542) ZP vs cUFHA_ZP AppendToGraph base vs cUFHA_ZP AppendToGraph fit_ZP vs fitX_ZP AppendToGraph basey vs basex AppendToGraph/R/T W vs cUFHA_W ModifyGraph margin(left)=44,margin(bottom)=44,margin(top)=5,margin(right)=44,gfSize=10 ModifyGraph gmSize=3,width=185,height=185 ModifyGraph mode(ZP)=3,mode(W)=3 ModifyGraph marker(ZP)=19,marker(base)=17,marker(fit_ZP)=16,marker(basey)=19,marker(W)=16 ModifyGraph lStyle(base)=3,lStyle(basey)=3 ModifyGraph rgb(ZP)=(65535,3855,13107),rgb(base)=(30583,30583,30583),rgb(fit_ZP)=(65535,3855,13107) ModifyGraph rgb(basey)=(34952,34952,34952),rgb(W)=(1,26221,39321) ModifyGraph msize(ZP)=3,msize(fit_ZP)=3 ModifyGraph useMrkStrokeRGB(ZP)=1,useMrkStrokeRGB(W)=1 ModifyGraph log(bottom)=1,log(right)=1,log(top)=1 ModifyGraph fSize(left)=10,fSize(bottom)=10 ModifyGraph btLen=3 ModifyGraph stLen=1 Label left "Zeta Potential (mV)" Label bottom "UFHA Concentration (mM)" Label right "Stability Ratio" SetAxis left -30,20 SetAxis bottom 0.01,10 SetAxis right 1,100 ErrorBars/RGB=(0,0,0) ZP Y,wave=(err_ZP,err_ZP) EndMacro Window Table0() : Table PauseUpdate; Silent 1 // building window... Edit/W=(-4.5,106.25,500.25,313.25) cUFHA_ZP,ZP,err_ZP as "W-KCl" ModifyTable format(Point)=1 EndMacro Window Table1() : Table PauseUpdate; Silent 1 // building window... Edit/W=(456.75,559.25,961.5,766.25) basex,basey as "base" ModifyTable format(Point)=1 EndMacro Window Table3() : Table PauseUpdate; Silent 1 // building window... Edit/W=(879.75,148.25,1384.5,355.25) cUFHA_EM,EM_UFHA,err_EM as "EM-UFHA-AL" ModifyTable format(Point)=1 EndMacro Window Table2() : Table PauseUpdate; Silent 1 // building window... Edit/W=(434.25,40.25,939,244.25) cUFHA_W,W as "W" ModifyTable format(Point)=1 EndMacro #pragma TextEncoding = "UTF-8" #pragma rtGlobals=3 // Use modern global access method and strict wave access #pragma DefaultTab={3,20,4} // Set default tab width in Igor Pro 9 and later