Instructions for Using CDPro:
There are 7 reference sets (basis sets) in CDPro, and an 8th one can be created using CLUSTER program. These can be selected for analysis with any of the three softwares, SELCON3, CDSSTR and CONTINLL, by changing the IBasis (Basis_1) value in the INPUT file. [IBasis and Basis_1 are the same]
 
 
All these programs and the output can now be accessed with ease in a browser environment (Netscape navigator, 4.7, frames compatible). Place the files in C:/CDPro directory and open RunCDPro.html in Navigator.

 
IBasis
(Basis_1)
Wavelength Range
Number of Proteins
Secondary Structures
1
178-260 nm
29
6 (aR, aD, bR, bD, T, U)
2
178-260 nm
22
6 (a, 3/10, b, T, P2, U)
3
185-240 nm
37
6 (aR, aD, bR, bD, T, U)
4
190-240 nm
43
6 (aR, aD, bR, bD, T, U)
5
185-240 nm
37*
5 (a, b, T, P2, U)
6
185-240 nm
42
6 (aR, aD, bR, bD, T, U)
7
190-240 nm
48
6 (aR, aD, bR, bD, T, U)
8 190-240 nm various 6 (aR, aD, bR, bD, T, U)

List of proteins for IBasis = 3 and 5 are same; the secondary structure fractions are different -- option 3 splits the helix and sheet fractions, while option5 gives P2.

Results from IBasis values 1, 3, 4, 6 and 7 are comparable as the secondary structure fractions they estimate are identical (From DSSP assignments with regular and distorted helix and sheet structures). IBasis = 2 uses assignments from XTLSSTR program of King and Johnson and IBasis = 5 uses different types of structures from DSSP assignments (helix, sheet, turns, poly(Pro)II, and unordered) of secondary structure; results from these two options cannot be combined with those from others.
IBasis = 8 uses tertiary class specific protein set. Use CLUSTER to create the required files before using this option.

To analyze your CD spectrum: